Application
Immunofluorescence staining of methanol-fixed Hep G2 cells showing membrane localization.Application
Western blot analysis of OSC expression in non-transfected 293T: (A), human OSC transfected 293T: (B) and HeLa (C) whole cell lysates.Application
Western blot analysis of OSC expression in non-transfected: (A) and human OSC transfected: (B) 293T whole cell lysates.| Product Name | OSC Antibody (N-20) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping near the N-terminus of OSC of human origin |
| Modification Notes | OSC, also known as LSS (Lanosterol synthase), is a 732 amino acid protein that contains 4 PFTB repeats and belongs to the terpene cyclase family. Functioning in the pathway of terpene metabolism, OSC catalyzes the first step in the biosynthesis of cholesterol, vitamin D and steroid hormones, namely the conversion of (S)-2,3 oxidosqualene to lanosterol. Lanosterol is a tetracyclic triterpenoid that is required for the synthesis of all steroids. Due to its role in lanosterol production, OSC is crucial for proper cholesterol formation and overall steroid function. Human OSC shares 83% homology with its rat counterpart, suggesting a conserved role between species. Multiple isoforms of OSC exist as a result of alternative splicing events. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of OSC of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine and bovine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping near the N-terminus of OSC of human origin |
|---|---|
| Tissue Specificity | epitope mapping near the N-terminus of OSC of human origin |
| Entrez Gene | 4047 |
|---|
Application
Immunofluorescence staining of methanol-fixed Hep G2 cells showing membrane localization.
Application
Western blot analysis of OSC expression in non-transfected 293T: (A), human OSC transfected 293T: (B) and HeLa (C) whole cell lysates.
Application
Western blot analysis of OSC expression in non-transfected: (A) and human OSC transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of OSC of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine and bovine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
