Application
Western blot analysis of PHKG1 expression in Ramos whole cell lysate.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human cancer tissue showing cytoplasmic staining.| Product Name | PHKG1 Antibody (I-17) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of PHKG1 of human origin by WB, IP, IF, IHC(P) and ELISA |
| Modification Notes | PHKG1 (Phosphorylase kinase subunit ©1), is a subunit of phosphorylase kinase (PHK) that belongs to the Ser/Thr protein kinase family. PHK is a hexadecameric protein composed of four å chains, four ∫ chains, four © chains and four ∂ chains. The © chains are catalytic chains, the å and ∫ chains are regulatory chains and the ∂ chains are calmodulins. PHKG1 contains two calmodulin-binding domains and one protein kinase domain. As the catalytic chain of PHK, PHKG1 is responsible for catalyzing the phosphorylation and activation of glycogen phosphorylase and therefore it plays an important role in the glycogenolytic pathway. Mutations in the gene encoding PHKG1 can lead to PHK deficiency and result in glycogen storage disease type 9C (GSD9C), also known as autosomal liver glycogenosis. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of PHKG1 of human origin by WB IP IF IHC(P) and ELISA |
|---|---|
| Tissue Specificity | recommended for detection of PHKG1 of human origin by WB, IP, IF, IHC(P) and ELISA |
| Entrez Gene | 5260 |
|---|
Application
Western blot analysis of PHKG1 expression in Ramos whole cell lysate.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human cancer tissue showing cytoplasmic staining.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
