Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human cancer tissue showing cytoplasmic staining.| Product Name | p-NBK Antibody (Thr 33) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of Thr 33 phosphorylated NBK of human origin by WB, IF, IHC(P) and ELISA |
| Modification | Phosphoralated |
| Modification Notes | The Bcl-2 gene was isolated at the chromosomal breakpoint of t-bearing follicular B cell lymphomas. Bcl-2 blocks cell death following a variety of stimuli and confers a death-sparing effect to certain hematopoietic cell lines following growth factor withdrawal. A protein designated Bax p21 (i.e., Bcl-associated X protein) has extensive amino acid homology with Bcl-2 and both homodimerizes and heterodimerizes with Bcl-2. Overexpression of Bax accelerates apoptotic death. Natural born killer (NBK), also known as Bik, is a protein that is functionally related to Bax, although the two proteins share very little sequence homology. NBK does not contain the conserved Bcl-2 homology domains (BH domains) characteristic of the Bcl-2 family. It does however, share nine amino acids with Bax in a region designated BH3, which may be the critical determinant for the protein’s death-promoting activities. Phosphorylation of NBK at Ser35 and Thr33 is required for eliciting efficient apoptotic activity. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIFIHCELISA |
| WB:1:500~1:2000 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of Thr 33 phosphorylated NBK of human origin by WB IF IHC(P) and ELISA |
|---|---|
| Tissue Specificity | recommended for detection of Thr 33 phosphorylated NBK of human origin by WB, IF, IHC(P) and ELISA |
| Entrez Gene | 638 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human cancer tissue showing cytoplasmic staining.| Application Notes | WB:1:500~1:2000 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
