Application
Western blot analysis of VILIP-3 expression in mouse cerebellum tissue extract.Application
Western blot analysis of VILIP-3 expression in non-transfected (A) and transfected (B) 293 whole cell lysates.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human cancer tissue showing cytoplasmic staining.| Product Name | VILIP-3 Antibody (N-25) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of VILIP-3 of mouse and human origin by WB, IP, IF, IHC(P) and ELISA |
| Modification Notes | The Visinin-like proteins, VILIP-1, VILIP-2 and VILIP-3, belong to a family of neuronal Ca2+ sensor (NCS) proteins conserved from yeast to human. Members of NCS family are mainly expressed in retinal photoreceptors, neuroendocrine cells or neurons and primarily function to regulate gene expression, modulate neurotransmitter release, direct regulation of ion channels, control cyclic nucleotide metabolism, biosynthesize polyphosphoinositides and participate in phototransduction. The NCS family is divided into five subfamilies, consisting of about 40 family members in total. Group III represents the VILIP family and includes hippocalcin, and neurocalcin-∂, along with VILIP-1, VILIP-2 and VILIP-3. Also known as Hippocalcin-like protein 1, VILIP-3 (Visinin-like protein 3) is a 193 amino acid protein that contains 4 EF-hand domians, which enable it to bind to two or three calcium ions. For this reason, it is likely that VILIP-3 is involved the the calcium-dependent regulation of rhodopsin phosphorylation. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of VILIP-3 of mouse and human origin by WB IP IF IHC(P) and ELISA |
|---|---|
| Tissue Specificity | recommended for detection of VILIP-3 of mouse and human origin by WB, IP, IF, IHC(P) and ELISA |
| Entrez Gene | 3241 |
|---|
Application
Western blot analysis of VILIP-3 expression in mouse cerebellum tissue extract.
Application
Western blot analysis of VILIP-3 expression in non-transfected (A) and transfected (B) 293 whole cell lysates.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human cancer tissue showing cytoplasmic staining.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
