Application
Western blot analysis of CLC-KB expression in rat kidney (A) and mouse kidney (B) tissue extracts.| Product Name | CLC-KB Antibody (C-18) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | raised against a peptide mapping at the C-terminus of CLC-KB of mouse origin |
| Modification Notes | The family of voltage-dependent chloride channels (CLCs) regulate cellular trafficking of chloride ions, a critical component of all living cells. CLCs regulate excitability in muscle and nerve cells, aid in organic solute transport, and maintain cellular volume (1). CLC-KA is a kidney-specific chloride channel that mediates transepithelial chloride transport in the thin ascending limb of the Henle loop in the inner medulla (2). CLC-KA plays a crucial role in urine concentration (2). The gene encoding human CLC-KA maps to chromosome 1p36 (3). Mutations in this gene may be associated with nephrogenic diabetes insipidus in those cases where mutations in the vasopressin V2 receptor and the AQP2 water channel are lacking (2). CLC-KB mediates basolateral chloride ion efflux in the thick ascending limb and in more distal nephron segments (4). The gene encoding human CLC-KB maps to chromosome 1p36 (3). Mutations in this gene cause type III Barter’s syndrome which is characterized by renal salt-wasting and low blood pressure (5). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHC |
| recommended for detection of CLC-KB of mouse, rat and human origin by WB, IP, IF and IHC(P): |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | raised against a peptide mapping at the C-terminus of CLC-KB of mouse origin |
|---|---|
| Tissue Specificity | raised against a peptide mapping at the C-terminus of CLC-KB of mouse origin |
| Entrez Gene | 1188 |
|---|
Application
Western blot analysis of CLC-KB expression in rat kidney (A) and mouse kidney (B) tissue extracts.| Application Notes | recommended for detection of CLC-KB of mouse, rat and human origin by WB, IP, IF and IHC(P): |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
