Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human heart muscle tissue showing cytoplasmic staining of myocytes.Application
Immunofluorescence staining of methanol-fixed HeLa cells showing membrane and cytoplasmic localization.Application
Western blot analysis of COL2A1 expression in HISM (A) and HT-1080 (B) whole cell lysates.Application
Western blot analysis of COL2A1 expression in non-transfected 293T: (A), mouse COL2A1 transfected 293T: (B) and HISM (C) whole cell lysates.Application
Western blot analysis of COL1A1 expression in non-transfected 293T: (A), mouse COL1A1 transfected 293T: (B) and Hela (C) whole cell lysates.| Product Name | COL2A1 Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 873-1072 mapping near the C-terminus of Collagen α1 Type II of human origin |
| Modification Notes | The extensive family of COL gene products (collagens) is composed of several chain types, including fibril-forming interstitial collagens (types I, II, III and V) and basement membrane collagens (type IV), each type containing multiple isoforms. Collagens are fibrous, extracellular matrix proteins with high tensile strength and are the major components of connective tissue, such as tendons and cartilage. All collagens contain a triple helix domain and frequently show lateral self-association in order to form complex connective tissues. Several collagens also play a role in cell adhesion, important for maintaining normal tissue architecture and function. In cartilage, Collagen Type II constitutes the bulk of the fibril. Sensitization with Collagen Type II induces an erosive polyarthritis in rats, mice and higher primates which can resemble rheumatoid arthritis and relapsing polychrondritis. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of Collagen α1 Type II, and to a lesser extent, Collagen α1 Type I of mouse, rat and human origin by WB, IP, IF IHC(P) and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 873-1072 mapping near the C-terminus of Collagen &alpha 1 Type II of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 873-1072 mapping near the C-terminus of Collagen α1 Type II of human origin |
| Entrez Gene | 1280 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human heart muscle tissue showing cytoplasmic staining of myocytes.
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing membrane and cytoplasmic localization.
Application
Western blot analysis of COL2A1 expression in HISM (A) and HT-1080 (B) whole cell lysates.
Application
Western blot analysis of COL2A1 expression in non-transfected 293T: (A), mouse COL2A1 transfected 293T: (B) and HISM (C) whole cell lysates.
Application
Western blot analysis of COL1A1 expression in non-transfected 293T: (A), mouse COL1A1 transfected 293T: (B) and Hela (C) whole cell lysates.| Application Notes | recommended for detection of Collagen α1 Type II, and to a lesser extent, Collagen α1 Type I of mouse, rat and human origin by WB, IP, IF IHC(P) and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
