Application
Western blot analysis of ZNF253 expression in non-transfected 293T: (A), human ZNF253 transfected 293T: (B) and Hep G2 (C) whole cell lysates.Application
Western blot analysis of ZNF253 expression in Hep G2 whole cell lysate.| Product Name | ZNF253 Antibody (F-16) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | raised against ZNF253 of human origin |
| Modification Notes | Zinc-finger proteins contain DNA-binding domains and have a wide variety of functions, most of which encompass some form of transcriptional activation or repression. The majority of zinc-finger proteins contain a Krüppel-type DNA binding domain and a KRAB domain, which is thought to interact with KAP1, thereby recruiting histone modifying proteins. ZNF253, also known as bone marrow zinc finger 1 (BMZF-1) or zinc finger protein 411, is a 275 amino acid protein belonging to the Krüppel C2H2-type zinc-finger protein family. Localized to the nucleus, ZNF253 contains one KRAB domain and three C2H2-type zinc fingers. Due to the presence of these domains, ZNF253 may be involved in transcriptional regulation. ZNF253 is also thought to have transcriptional repression activity. ZNF253 is expressed in immature erythroid cell lines and in bone marrow. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPELISA |
| recommended for detection of ZNF253 of human origin by WB, IP and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | raised against ZNF253 of human origin |
|---|---|
| Tissue Specificity | raised against ZNF253 of human origin |
| Entrez Gene | 56242 |
|---|
Application
Western blot analysis of ZNF253 expression in non-transfected 293T: (A), human ZNF253 transfected 293T: (B) and Hep G2 (C) whole cell lysates.
Application
Western blot analysis of ZNF253 expression in Hep G2 whole cell lysate.| Application Notes | recommended for detection of ZNF253 of human origin by WB, IP and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
