Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human lung carcinoma tissue showing cytoplasmic staining.| Product Name | p-PKC ζ Antibody (Thr 410) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to phosphorylated Thr 410 of PKC ζ of human origin |
| Modification | Phosphoralated |
| Modification Notes | Members of the protein kinase C (PKC) family play a key regulatory role in a variety of cellular functions, including cell growth and differentiation, gene expression, hormone secretion and membrane function. PKCs were originally identified as serine/threonine protein kinases whose activity was dependent on calcium and phospholipids. Diacylglycerols (DAG) and tumor promoting phorbol esters bind to and activate PKC. PKCs can be subdivided into at least two major classes, including conventional (c) PKC isoforms (å, ∫I, ∫II and ©) and novel (n) PKC isoforms (∂, é , Ω, h and †). Upon phosphorylation on Thr-507, PKC ∂ is activated, where it can inhibit the functionality of specific substrates, such as Jak2 and Stat3. PKC ∂ phosphorylates and associates with Stat3 on Ser-727 following induction by IL-6 to negatively regulate the DNA binding and transcriptional activity of Stat3. Phosphorylation of PKC Ω is induced by PDK1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IFIHC |
| recommended for detection of Thr 410 phosphorylated PKC ζ of mouse, rat and human origin by IF and IHC(P): |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to phosphorylated Thr 410 of PKC &zeta of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to phosphorylated Thr 410 of PKC ζ of human origin |
| Entrez Gene | 5590 |
|---|
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human lung carcinoma tissue showing cytoplasmic staining.| Application Notes | recommended for detection of Thr 410 phosphorylated PKC ζ of mouse, rat and human origin by IF and IHC(P): |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
