Application
Western blot analysis of GCL expression in mouse testis tissue extract.| Product Name | GCL Antibody (H-60) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 457-511 mapping near the C-terminus of GCL of human origin |
| Modification Notes | Germ cell-less (GCL) represses transcription during the later stages of spermatogenesis both in Drosophila and mammalian species. GCL localizes to the nuclear envelope, where it competes with BAF to interact with emerin, a complex that is required for appropriate gene expression. Defective sperm motility as well as impaired nuclear envelope integrity in liver, pancreas and testis cells result from a lack of GCL gene expression. Thus it appears that the essential function of GCL is to facilitate normal nuclear-lamina organization, which results in normal sperm morphogenesis and chromatin remodeling. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of GCL of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 457-511 mapping near the C-terminus of GCL of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 457-511 mapping near the C-terminus of GCL of human origin |
| Entrez Gene | 64396 |
|---|
Application
Western blot analysis of GCL expression in mouse testis tissue extract.| Application Notes | recommended for detection of GCL of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
