Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human heart tissue showing cytoplasmic staining of myocytes.Application
Western blot analysis of SEMA3A expression in rat brain tissue extract.| Product Name | SEMA3A Antibody (H-130) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 642-771 mapping at the C-terminus of Semaphorin 3A of human origin |
| Modification Notes | Semaphorins are a family of cell surface and secreted proteins that are conserved from insects to humans. Members of this family of proteins are approximately 750 amino acids in length (including signal sequences) and are defined by a conserved extracellular “semaphorin” domain of approximately 500 amino acids containing 14-16 cysteines, blocks of conserved sequences and no obvious repeats. Secreted and cell-bound semaphorins chemically attract and repel the growth of neural axons, guiding the development of intricate networks of neural tissue. SEMA3A (semaphorin-3A), also known as SEMA1, SEMAD, SEMAL, coll-1, Hsema-I, SEMAIII or Hsema-III, is a 771 amino acid secreted protein that belongs to the semaphorin family and can function as both a chemoattractive agent or a chemorepulsive agent. SEMA3A binds neuropilin and is able to induce the collapse and paralysis of neuronal growth cones. SEMA3A contains one immunoglobulin-like (Ig-like) domain, one PSI domain and one semaphorin domain. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of precursor and mature forms of SEMA3A and, to a lesser extent, SEMA3B of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 642-771 mapping at the C-terminus of Semaphorin 3A of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 642-771 mapping at the C-terminus of Semaphorin 3A of human origin |
| Entrez Gene | 10371 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human heart tissue showing cytoplasmic staining of myocytes.
Application
Western blot analysis of SEMA3A expression in rat brain tissue extract.| Application Notes | recommended for detection of precursor and mature forms of SEMA3A and, to a lesser extent, SEMA3B of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
