Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue showing nuclear staining.| Product Name | p-HDAC8 Antibody (Ser 39) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to phosphorylated Ser 39 of HDAC8 of human origin |
| Modification | Phosphoralated |
| Modification Notes | In the intact cell, DNA closely associates with histones and other nuclear proteins to form chromatin. The remodeling of chromatin is believed to be a critical component of transcriptional regulation and a major source of this remodeling is brought about by the acetylation of nucleosomal histones. Acetylation of lysine residues in the amino-terminal tail domain of histone results in an allosteric change in the nucleosomal conformation and an increased accessibility to transcription factors by DNA. Conversely, the deacetylation of histones is associated with transcriptional silencing. Several mammalian proteins have been identified as nuclear histone acetylases, including GCN5, PCAF (p300/CBP-associated factor), p300/CBP, HAT1 and the TFIID subunit TAF II p250. Mammalian HDAC8, isolated from human kidney, is a histone deacetylase that shares homology to other HDACs but has different tissue distribution. HDAC8 is localized to the nucleus and plays a role in the development of a broad range of tissues and in the etiology of cancer. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IFIHC |
| recommended for detection of Ser 39 phosphorylated HDAC8 of mouse, rat and human origin by IF and IHC(P): |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to phosphorylated Ser 39 of HDAC8 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to phosphorylated Ser 39 of HDAC8 of human origin |
| Entrez Gene | 55869 |
|---|
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue showing nuclear staining.| Application Notes | recommended for detection of Ser 39 phosphorylated HDAC8 of mouse, rat and human origin by IF and IHC(P): |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
