Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human cervix tissue showing cytoplasmic staining of squamous epithelial cells.Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.Application
Western blot analysis of FLIPS/L expression in non-transfected 293T: (A), human FLIPS/L transfected 293T: (B) and SW480 (C) whole cell lysates.Application
Western blot analysis of FLIPS/L expression in non-transfected: (A) and mouse FLIPS/L transfected: (B) 293T whole cell lysates.Application
Western blot analysis of FLIP expression in Jurkat whole cell lysate.| Product Name | FLIPS/L Antibody (H-202) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-202 mapping at the N-terminus of FLIP of human origin |
| Modification Notes | FLIP (FLICE inhibitory protein) is expressed as both long and short forms and is involved in the regulation of apoptosis. The short form of FLIP contains two death effector domains homologous to the death effector domain of the FAS-associating protein FADD. The long form of FLIP, which shares significant homology with the cysteine protease FLICE, contains an additional caspase-like domain, but lacks a catalytic active site and lacks the residues that form the substrate binding pocket in most caspases. FLIP has been designated by independent groups as Casper, I-FLICE, CLARP, FLAME-1 and MRIT. Although its exact role is still being elucidated, FLIP appears to be an important factor in the regulation of apoptosis downstream of all known death receptors. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of FLIPS and FLIPL of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-202 mapping at the N-terminus of FLIP of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-202 mapping at the N-terminus of FLIP of human origin |
| Entrez Gene | 8837 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human cervix tissue showing cytoplasmic staining of squamous epithelial cells.
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.
Application
Western blot analysis of FLIPS/L expression in non-transfected 293T: (A), human FLIPS/L transfected 293T: (B) and SW480 (C) whole cell lysates.
Application
Western blot analysis of FLIPS/L expression in non-transfected: (A) and mouse FLIPS/L transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of FLIP expression in Jurkat whole cell lysate.| Application Notes | recommended for detection of FLIPS and FLIPL of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
