Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human pancreas tissue showing cytoplasmic staining of Islets of Langerhans.Application
Western blot analysis of Chr-A expression in non-transfected 293T: (A), human Chr-A transfected 293T: (B) and Hep G2 (C) whole cell lysates.Application
Western blot analysis of Chr-A expression in non-transfected 293T: (A), human Chr-A transfected 293T: (B) and Hep G2 (C) whole cell lysates.Application
Immunofluorescence staining of normal mouse liver frozen section showing extracellular staining.Application
Western blot analysis of Chr-A expression in nocodazole-treated SH-SY5Y whole cell lysate.Application
Western blot analysis of purified Chr-A of cow origin.| Product Name | Chr-A Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 158-457 mapping at the C-terminus of chromogranin A of human origin |
| Modification Notes | Chromogranins (secretogranins) are acidic glycoproteins that localize within secretory granules of endocrine, neuroendocrine and neuronal tissue. Family members include chromogranin A (Chr-A), chromogranin B (Chr-B, also known as secretogranin I) chromogranin C (also known as secretogranin II or Sg II), secretogranin III (Sg III or SCG3). High levels of Chr-A expression is a characteristic of neuroendocrine tumors. Pancreastatin is a peptide derived from Chr-A which inhibits insulin secretion, exocrine pancreatic secretion and gastric acid secretion. Pancreastatin exists as two forms; the major form is expressed in stomach and colon extracts. In neuroendocrine cells the level Sg II has been shown to increase four-fold in response to histamine, while levels of Chr-A and Chr-B showed little or no increase. Sg III is an acidic secretory protein expressed in neuronal and endocrine cells. In the anterior lobe of the rat pituitary gland, Sg III is present in mammotropes and thyrotropes, moderately in gonadotropes and corticotropes, though not in somatotropes. Sg III and carboxypeptidase E (CPE) bind specifically to cholesterol-rich secretory granule (SG) membranes. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of chromogranin A of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 158-457 mapping at the C-terminus of chromogranin A of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 158-457 mapping at the C-terminus of chromogranin A of human origin |
| Entrez Gene | 1113 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human pancreas tissue showing cytoplasmic staining of Islets of Langerhans.
Application
Western blot analysis of Chr-A expression in non-transfected 293T: (A), human Chr-A transfected 293T: (B) and Hep G2 (C) whole cell lysates.
Application
Western blot analysis of Chr-A expression in non-transfected 293T: (A), human Chr-A transfected 293T: (B) and Hep G2 (C) whole cell lysates.
Application
Immunofluorescence staining of normal mouse liver frozen section showing extracellular staining.
Application
Western blot analysis of Chr-A expression in nocodazole-treated SH-SY5Y whole cell lysate.
Application
Western blot analysis of purified Chr-A of cow origin.| Application Notes | recommended for detection of chromogranin A of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
