Application
Western blot analysis of Lefty-A expression in non-transfected: (A) and human Lefty-A transfected: (B) 293T whole cell lysates and rat testis tissue extract (C).Application
Western blot analysis of Lefty expression in non-transfected: (A) and human Lefty transfected: (B) 293T whole cell lysates.| Product Name | Lefty Antibody (H-290) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 77-366 mapping within the mature chain of Lefty of human origin |
| Modification Notes | The TGF∫ superfamily is composed of numerous growth and differentiation factors, including transforming growth factor (TGF) 1, 2 and 3; growth/differ-entiation factor (GDF) 1-9; Mullerian inhibiting substance (MIS); bone morpho-genic protein (BMP) 2-8; glial cell line-derived neurotrophic factor (GDNF); inhibins a, b-A, b-B and b-C; Lefty and Nodal. Members of the TGF superfamily are involved in embryonic development and adult tissue homeostasis. Lefty-A and Lefty-B are homologues of murine Lefty-1 and Lefty-2. Lefty-1 is required for left-right axis determination as a regulator of Lefty-2 and Nodal. It is a secreted protein expressed on the left side of developing embryos. The expression of Lefty-1 is mostly in the prospective floor plate (PFP), although weak expression can be seen in the lateral-plate mesoderm (LPM). It is involved in establishing left-right asymmetry of the organ systems of mammals. Lefty-A plays a role in endometrial bleeding. Mutations in this gene have been associated with left-right axis malformations, particularly in the heart and lungs. Some types of infertility have been associated with dysregulated expression of this gene in the endometrium. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of precursor and mature Lefty-A and Lefty-B of human origin and Lefty-1 and Lefty-2 of mouse and rat origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 77-366 mapping within the mature chain of Lefty of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 77-366 mapping within the mature chain of Lefty of human origin |
| Entrez Gene | 7044 |
|---|
Application
Western blot analysis of Lefty-A expression in non-transfected: (A) and human Lefty-A transfected: (B) 293T whole cell lysates and rat testis tissue extract (C).
Application
Western blot analysis of Lefty expression in non-transfected: (A) and human Lefty transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of precursor and mature Lefty-A and Lefty-B of human origin and Lefty-1 and Lefty-2 of mouse and rat origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
