Application
Antibodies tested include p-AMPKα1/2 (Thr 172): (A,C) and AMPKα1 (71.54): (B,D).| Product Name | p-AMPKα1/2 Antibody (Thr 172) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to phosphorylated Thr 172 of AMPKα2 of human origin |
| Modification | Phosphoralated |
| Modification Notes | Five-prime-AMP-activated protein kinase, known as AMPK, is a heterotrimeric complex that comprises of a catalytic å subunit, and regulatory ∫ and ©. AMPK protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. AMPK is activated by high AMP and low ATP via a mechanism involving allosteric regulation, promotion of phosphorylation by an upstream protein kinase known as AMPK kinase (AMPKK), and inhibition of dephosphorylation. Activated AMPK can phosphorylate and regulate in vivo hydroxymethylglutaryl-CoA reductase and acetyl-CoA carboxylase, which are key regulatory enzymes of sterol synthesis and fatty acid synthesis, respectively. The human AMPKå1 gene maps to chromosome 5p12 and encodes a 548 amino acid protein. The major regulatory site phosphorylated by AMPKK on AMPKå has been identified as Thr 172 within the activation loop between the DFG and APE motifs of the alpha-subunits. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Thr 172 phosphorylated AMPK α1 and α2 isoforms of the catalytic subunit of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to phosphorylated Thr 172 of AMPK&alpha 2 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to phosphorylated Thr 172 of AMPKα2 of human origin |
| Entrez Gene | 5562 |
|---|
Application
Antibodies tested include p-AMPKα1/2 (Thr 172): (A,C) and AMPKα1 (71.54): (B,D).| Application Notes | recommended for detection of Thr 172 phosphorylated AMPK α1 and α2 isoforms of the catalytic subunit of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
