Application
Western blot analysis of His-tagged human recombinant GADD 45α.Application
Antibodies tested include GADD 45α (C-20): (A) and GADD 45α (H-165): (B).Application
Immunofluorescence staining of methanol-fixed GADD 45α-transfected COS cells showing nuclear localization.| Product Name | GADD 45α Antibody (H-165) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-165 representing full length GADD 45α of human origin |
| Modification Notes | It is well established that cell cycle progression is subject to arrest at G1 and G2 checkpoints in response to DNA damage, presumably to allow time for DNA repair prior to entry into S and M phase, respectively. The p53 tumor suppressor is required for one such G1 checkpoint and functions to upregulate expression of GADD 45 and p21. p21 functions to inhibit the kinase activity of multiple Cdk complexes, which may account for its suppression of cell growth. GADD 45 binds both Cdks and PCNA, a protein involved in DNA replication and repair. GADD 45 has been shown to stimulate DNA excision repair in vitro and to inhibit entry of cells into S phase. Thus, it has been suggested that GADD 45 may serve as a link between p53-dependent cell cycle checkpoint and DNA repair . |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISAChIP |
| recommended for detection of GADD 45α and, to a lesser extent, GADD 45β and GADD 45γ of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-165 representing full length GADD 45&alpha of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-165 representing full length GADD 45α of human origin |
| Entrez Gene | 1647 |
|---|
Application
Western blot analysis of His-tagged human recombinant GADD 45α.
Application
Antibodies tested include GADD 45α (C-20): (A) and GADD 45α (H-165): (B).
Application
Immunofluorescence staining of methanol-fixed GADD 45α-transfected COS cells showing nuclear localization.| Application Notes | recommended for detection of GADD 45α and, to a lesser extent, GADD 45β and GADD 45γ of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
