Application
Western blot analysis of μ-protocadherin expression in non-transfected: (A) and human μ-protocadherin transfected: (B) 293T whole cell lysates.Application
Western blot analysis of μ-protocadherin expression in non-transfected: (A) and mouse μ-protocadherin transfected: (B) 293T whole cell lysates.| Product Name | μ-protocadherin Antibody (H-225) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 126-350 mapping within an extracellular domain of μ-protocadherin of human origin |
| Modification Notes | The mucin-like protocadherin, µ-protocadherin, is a developmentally regulated, single pass type I transmembrane protein that belongs to the cadherin superfamily. It contains four cadherin-like ectodomains, a triply repeating mucin domain, four SH3 binding regions, N- and O-glycosylation sites and a possible C-terminal PDZ binding domain. µ-protocadherin is expressed in various epithelial tissues and localizes to the apical surface along the brush border of the proximal convoluted tubule. It acts as a calcium dependent cell adhesion molecule mediating cell aggregation and may play a role in the activation of signaling events. Due to alternative splicing at least four isoforms exist for µ-protocadherin. These isoforms vary in the region containing the mucin-like domains. Only the longest isoform contains the triply repeating mucin domain. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of μ-protocadherin of human and, to a lesser extent, mouse and rat origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 126-350 mapping within an extracellular domain of &mu -protocadherin of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 126-350 mapping within an extracellular domain of μ-protocadherin of human origin |
| Entrez Gene | 53841 |
|---|
Application
Western blot analysis of μ-protocadherin expression in non-transfected: (A) and human μ-protocadherin transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of μ-protocadherin expression in non-transfected: (A) and mouse μ-protocadherin transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of μ-protocadherin of human and, to a lesser extent, mouse and rat origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
