| Product Name | NEDD1 Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 61-360 mapping near the N-terminus of NEDD1 of human origin |
| Modification Notes | NEDD1 (neural precursor cell expressed, developmentally down-regulated 1), also known as GCP-WD, is a homolog of the Drosophila protein known as Dgp71WD. It is a ubiquitously expressed, evolutionarily conserved protein and contains eight WD40 repeats and a coiled coil domain at the C-terminus. NEDD1 is a subunit of the ©-tubulin ring complex (©TuRC) and plays an important role in mitosis. During mitosis NEDD1 is phosphorylated and functions in forming the association of ©-tubulin with the spindle. The state of phosphorylation of NEDD1 is also important for determining its cellular localization. NEDD1 is responsible for targeting ©TuRC to the centrosome and spindle and is therefore required for centrosomal and chromatin-mediated microtubule nucleation. The inhibition of NEDD1 results in the loss of ©TuRC from the centrosome and a sequential loss of microtubule nucleation. Due to its critical role in mitosis, NEDD1 may be a potential target for anticancer therapies. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of NEDD1 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 61-360 mapping near the N-terminus of NEDD1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 61-360 mapping near the N-terminus of NEDD1 of human origin |
| Entrez Gene | 121441 |
|---|
| Application Notes | recommended for detection of NEDD1 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
