| Product Name | Rho1 Antibody (y-260) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-150 mapping at the N-terminus of Rho1 of Saccharomyces cerevisiae origin |
| Modification Notes | In Saccharomyces cerevisiae, the small GTPase Rho1 plays an essential role in the control of cell wall synthesis and organization of the actin cytoskeleton (1). In the budding yeast S. cerevisiae, one of the main structural components of the cell wall is 1,3-beta-glucan produced by 1,3-beta-glucan synthase (GS) (2). Rho1 is required for 1,3-beta-glucan synthase activity, as yeast GS is composed of a putative catalytic subunit encoded by FKS1 and FKS2 and a regulatory subunit encoded by RHO1 (2,3). Rho1 is also required for activation of protein kinase C and the cell integrity pathway, as well as for progression into G1, cell polarization and exocytosis (3). Activation of PKC1 occurs via the GTPase RHO1 and the kinase pair PKH1 and PKH2 (4). Several regulators for Rho1 are known, including the GTPase-activating proteins (GAPs) SAC7, BEM2 and BAG7 (1). Rho1 directs formin-mediated actin ring assembly during budding yeast cytokinesis (5). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPELISA |
| recommended for detection of Rho1 of Saccaromyces cerevisiae origin by WB, IP and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-150 mapping at the N-terminus of Rho1 of Saccharomyces cerevisiae origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-150 mapping at the N-terminus of Rho1 of Saccharomyces cerevisiae origin |
| Application Notes | recommended for detection of Rho1 of Saccaromyces cerevisiae origin by WB, IP and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
