Application
Western blot analysis of Neuregulin-1α/β1/ expression in A-431 (A), MCF7 (B), MDA-MB-231 (C), SK-BR-3 (D) and HeLa (E) whole cell lysates.Application
Staining of methanol-fixed 3611-RF cells showing cytoplasmic immunostaining and nuclear DAPI counterstain.Application
Western blot analysis of Neuregulin-1α/β1/2 expression in THP-1 (A), A-431 (B) and MCF7 (C) whole cell lysates.| Product Name | Neuregulin-1α/β1/2 Antibody (C-20) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping at the C-terminus of Neuregulin-1 isoform HRG-α of human origin |
| Modification Notes | The neuregulins are a family of ErbB/HER ligands encoded by four genes. Neuregulin-1 gene, NRG-1, encodes numerous splice variants with differing transcription initiation sites. Neuregulin-1 includes a range of isoforms with varying glycosylation, regulation of expression and function. Neuregulin-1 splice variants each bear an EGF-like domain, though, otherwise have unique domain structures, differing functions and discrete tissue distribution. Six types of Neuregulin-1 isoform groups have been defined based on their structural features. Three types are most often described, type I (ARIA, NDF, or HRG), type II (GGF) and type III (SMDF). Neuregulin-1 has been linked to schizophrenia and has diverse neural functions. Neuregulin-1 affects cell migration, the differentiation of neural crest and Schwann cells and acts to upregulate the expression of acetylcholine receptors at muscle fibers during the formation of neuromuscular junctions. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Neuregulin-1 isoforms HRG-α, HRG-α1A, HRG-β1 and HRG-β2 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping at the C-terminus of Neuregulin-1 isoform HRG-&alpha of human origin |
|---|---|
| Tissue Specificity | epitope mapping at the C-terminus of Neuregulin-1 isoform HRG-α of human origin |
| Entrez Gene | 3084 |
|---|
Application
Western blot analysis of Neuregulin-1α/β1/ expression in A-431 (A), MCF7 (B), MDA-MB-231 (C), SK-BR-3 (D) and HeLa (E) whole cell lysates.
Application
Staining of methanol-fixed 3611-RF cells showing cytoplasmic immunostaining and nuclear DAPI counterstain.
Application
Western blot analysis of Neuregulin-1α/β1/2 expression in THP-1 (A), A-431 (B) and MCF7 (C) whole cell lysates.| Application Notes | recommended for detection of Neuregulin-1 isoforms HRG-α, HRG-α1A, HRG-β1 and HRG-β2 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
