Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human cerebellum tissue showing cytoplasmic staining of Purkinje cells and cells in granular layers (low and high magnification). Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Western blot analysis of A cyclase V/VI expression in SK-N-SH (A) and C6 (B) whole cell lysates.| Product Name | A cyclase V/VI Antibody (C-17) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping at the C-terminus of A cyclase V/VI of human origin |
| Modification Notes | Adenylyl cyclases function to convert ATP to cyclic AMP in response to activation by a variety of hormones, neurotransmitters and other regulatory molecules (1,2). Cyclic AMP, in turn, activates several other target molecules to control a broad range of diverse phenomena such as metabolism, gene transcription and memory (3). Adenylyl cyclases respond to receptor-initiated signals, mediated by the Gs and Gi heterotrimeric G proteins. The binding of an agonist to a Gs-coupled receptor catalyzes the exchange of GDP (bound to Gå s) for GTP, the dissociation of GTP-Gå s from G∫© and Gå s-mediated activation of adenylyl cyclase (3,4). Adenylyl cyclases V (AC V) and VI (AC VI) have multiple messages (5). AC V and AC VI are highly expressed in heart. Unlike AC VI, AC V is expressed to a lesser extent in brain and is absent in a variety of other tissues (6). Both AC V and AC VI can be stimulated by NaF, guanosine 5’-[©-thio]triphosphoate and forskolin but not by Ca2+/calmodulin (5-7). Activation of the D2 dopaminergic and m4 muscarine receptors inhibit the activity of adenylyl cyclase isozymes I, V, VI and VIII, whereas type II, IV and VII are stimulated and type III is not affected (8). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of adenylyl cyclases V and VI and, to a lesser extent, adenylyl cyclase I of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping at the C-terminus of A cyclase V/VI of human origin |
|---|---|
| Tissue Specificity | epitope mapping at the C-terminus of A cyclase V/VI of human origin |
| Entrez Gene | 111 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human cerebellum tissue showing cytoplasmic staining of Purkinje cells and cells in granular layers (low and high magnification). Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Western blot analysis of A cyclase V/VI expression in SK-N-SH (A) and C6 (B) whole cell lysates.| Application Notes | recommended for detection of adenylyl cyclases V and VI and, to a lesser extent, adenylyl cyclase I of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
