Application
Western blot analysis of RXRα expression in non-transfected: (A) and human RXRα transfected: (B) 293T whole cell lysates.Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization.Application
Western blot analysis of 10 ng of purified human RXRβ.| Product Name | RXR Antibody (ΔN 197) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 198-462 representing the complete ligand binding domain of RXRα of human origin |
| Conjugation | Agarose |
| Modification | Acetylated |
| Modification Notes | Retinoids are metabolites of vitamin A (retinol) and are believed to represent important signaling molecules during vertebrate development and tissue differentiation. Two families of retinoid receptors have been identified. Retinoic acid receptors (RARs) include RARå, RAR∫ and RAR©, each of which have a high affinity for all transretinoic acids and belong to the same class of nuclear transcription factors as thyroid hormone receptors, vitamin D3 receptor and ecdysone receptor. The ligand-binding domains of the RARs are highly conserved and RAR isoforms are expressed in distinct patterns throughout development and in the mature organism. Members of the retinoid X receptor (RXR) family, RXRå, RXR∫ and RXRg, are activated by 9-cis-RA, a stero- and photoisomer of all trans-RA that is expressed in vivo in both liver and kidney and may represent a widely used hormone. As is true for the RAR subfamily, the RXR receptors are closely related to each other both in their DNA-binding and ligand-binding domains and are encoded by separate genes at distinct chromosomal loci. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ChIPELISAGSIFIPWB |
| WB:1:100~1:500 IP:1:20~1:100 IF:1:20~1:100 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 198-462 representing the complete ligand binding domain of RXRα of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 198-462 representing the complete ligand binding domain of RXRα of human origin |
| Entrez Gene | 6256 |
|---|
Application
Western blot analysis of RXRα expression in non-transfected: (A) and human RXRα transfected: (B) 293T whole cell lysates.
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization.
Application
Western blot analysis of 10 ng of purified human RXRβ.| Application Notes | WB:1:100~1:500 IP:1:20~1:100 IF:1:20~1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
