| Product Name | p-αPAK Antibody (Ser 199/204) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to dually phosphorylated Ser 199 and Ser 204 of αPAK of human origin |
| Modification | Phosphoralated |
| Modification Notes | p21-activated kinases (PAK) are serine/threonine kinases that link Rho GTPases to cytoskeletal reorganization and nuclear signaling. Three common isoforms are åPAK p68, ∫PAK p65 and ©PAK p62. å, ∫ and ©PAK isoforms associate with Rac 1 and Cdc42 in their active GTP-bound state, inhibiting their intrinsic GTPase activity and mediating their autophosphorylation. ©PAK can undergo phosphorylation on Ser-19, Ser-141 and Thr-402, and phosphorylation of Ser-141 and Thr-402 correlates with ©PAK activation. Autophosphorylation of åPAK Thr-423 (Thr-402 for ∫PAK and Thr-421 for ©PAK) is catalyzed by Cdc42 and is required for kinase activation of PAK. Once phosphorylated and their affinity for Rac/Cdc42 reduced, PAK isoforms disassociate from the complex to seek downstream substrates. One such substrate is MEK kinase, an upstream effector of MEK4 which is involved in the JNK signaling pathway. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Ser 199 and Ser 204 phosphorylated αPAK of mouse, rat and human origin by WB, IP, IF and ELISA may cross-react with correspondingly phosphorylated βPAK and γPAK also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to dually phosphorylated Ser 199 and Ser 204 of &alpha PAK of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to dually phosphorylated Ser 199 and Ser 204 of αPAK of human origin |
| Entrez Gene | 5058 |
|---|
| Application Notes | recommended for detection of Ser 199 and Ser 204 phosphorylated αPAK of mouse, rat and human origin by WB, IP, IF and ELISA may cross-react with correspondingly phosphorylated βPAK and γPAK also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
