Application
Western blot analysis of Hemopexin expression in human liver tissue extract.| Product Name | Hemopexin Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 163-462 mapping at the C-terminus of Hemopexin of human origin |
| Modification Notes | Hemopexin (also known as ∫ 1B glycoprotein or HPX), a 462 amino acid protein, funtions as a scavenger and transporter of toxic plasma heme, transporting heme to the liver for breakdown and iron recovery. Hemopexin cooperates with Albumin, Haptoglobin, and high and low density lipoproteins to trap toxic plasma heme, which occurs as the result of the degradation of hemoglobin, myoglobin and enzymes with heme prosthetic groups, and to ensure the clearance of toxic heme from the plasma. After releasing the heme molecule, the free Hemopexin returns to circulation. It is expressed by the liver and is secreted in plasma. Hemopexin may play a role in the maintenance of metal ion homeostasis. It binds the following metal ions in order of highest to lowest affinity: nickel, copper, cobalt, zinc and manganese. Hemopexin can also act as a toxic protease that leads to proteinuria and glomerular alterations, which are characteristics of minimal changes disease (MCD), a common cause of nephrotic syndrome. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Hemopexin of mouse, rat and human origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 163-462 mapping at the C-terminus of Hemopexin of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 163-462 mapping at the C-terminus of Hemopexin of human origin |
Application
Western blot analysis of Hemopexin expression in human liver tissue extract.| Application Notes | recommended for detection of Hemopexin of mouse, rat and human origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
