Application
Western blot analysis of Protamine 2 expression in non-transfected: (A) and human Protamine 2 transfected: (B) 293T whole cell lysates.| Product Name | Protamine 2 Antibody (FL-102) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-102 representing full length Protamine 2 of human origin |
| Modification Notes | Protamines are small, arginine-rich (basic), nuclear proteins that mediate normal sperm head condensation and DNA stabilization (1–3). Mice, humans, and certain fish have 2 or more different protamines, whereas the sperm of bull, boar, rat, rabbit, guinea pig, and ram have one form of protamine (3,4). The majority of DNA in human sperm is bound to protamines with only a small proportion of DNA bound to histones in a way similar to active chromatin (1,2). The retention of histone association with sperm DNA with respect to protamine association to sperm DNA can change within as little as 400 bp of DNA, suggesting that there is fine control over haploid DNA organization (1). Protamines eventually replace histones late in the haploid phase of spermatogenesis (1,2). The human protamine 1 gene maps to chromosome 16p13.13 and encodes a 51 amino acid protein (5). The human protamine 2 gene maps to chromosome 16p13.13 and encodes a 102 amino acid protein (6). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Protamine 2 of human origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-102 representing full length Protamine 2 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-102 representing full length Protamine 2 of human origin |
| Entrez Gene | 5620 |
|---|
Application
Western blot analysis of Protamine 2 expression in non-transfected: (A) and human Protamine 2 transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of Protamine 2 of human origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
