Application
Western blot analysis of ML-IAP expression in H4 (A), SK-MEL-28 (B), HeLa (C) and A-431 (D) whole cell lysates.| Product Name | ML-IAP Antibody (H-90) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 151-240 mapping within an internal region of ML-IAP of human origin |
| Modification Notes | Inhibitor of apoptosis proteins (IAPs) contain conserved, unique N-terminal baculovirus IAP repeats (BIRs) and usually a C-terminal RING finger domain. Immunoprecipitation and Western blot analysis indicate that ML-IAP, also known as melanoma inhibitor of apoptosis protein, kidney inhibitor of apoptosis protein (KIAP), livin or BIRC7, binds to caspase-3, -7 and -9, but only inhibits caspase-9. Additionally, ML-IAP physically interacts with Smac through its BIR domain with a very high affinity and this interaction is very specific. The gene which encodes ML-IAP maps to human chromosome 20q13.3. There is controversy regarding the localization of this protein and its involvement in apoptosis, but it has been suggested that ML-IAP may play a complex role in the regulation of apoptosis. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of ML-IAP isoforms 1-3 of human origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 151-240 mapping within an internal region of ML-IAP of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 151-240 mapping within an internal region of ML-IAP of human origin |
| Entrez Gene | 79444 |
|---|
Application
Western blot analysis of ML-IAP expression in H4 (A), SK-MEL-28 (B), HeLa (C) and A-431 (D) whole cell lysates.| Application Notes | recommended for detection of ML-IAP isoforms 1-3 of human origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
