Application
Antibodies tested include p-Histone H2B (Ser 14): (A,B,C) and Histone H2B (FL-126): (D,E,F).| Product Name | p-Histone H2B Antibody (Ser 14) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of Ser 14 phosphorylated Histone H2B of mouse, rat and human origin by WB, IP, IF and ELISA |
| Modification | Phosphoralated |
| Modification Notes | In eukaryotes, DNA is wrapped around histone octamers to form the basic unit of chromatin structure. The octamer is composed of histones H2A, H2B, H3 and H4 and it associates with approximately 200 base pairs of DNA to form the nucleosome. The association of DNA with histones results in dense packing of chromatin, which restricts proteins involved in gene transcription from binding to DNA. Histone H2B, which is not usually phosphorylated in quiescent or growing cells, is phosphorylated after treatment with various apoptotic inducers. Histone H2B phosphorylation occurs universally in apoptotic cells and is associated with apoptosis-specific nucleosomal DNA fragmentation making it a biochemical hallmark of apoptotic cells. Phosphorylation of Histone H2B at serine 14 correlates with cells undergoing programmed cell death in vertebrates. Mst1 (mammalian sterile twenty kinase) can phosphorylate H2B at Ser 14 in vitro and in vivo, and the onset of H2B Ser 14 phosphorylation is dependent upon cleavage of Mst1 by caspase-3. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| blocking peptide, sc-31671 P: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of Ser 14 phosphorylated Histone H2B of mouse rat and human origin by WB IP IF and ELISA |
|---|---|
| Tissue Specificity | recommended for detection of Ser 14 phosphorylated Histone H2B of mouse, rat and human origin by WB, IP, IF and ELISA |
Application
Antibodies tested include p-Histone H2B (Ser 14): (A,B,C) and Histone H2B (FL-126): (D,E,F).| Application Notes | blocking peptide, sc-31671 P: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
