Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.Application
Intracellular FCM analysis of fixed and permeabilized Jurkat cells. Black line histogram represents the isotype control, normal rabbit IgG: .Application
Antibodies tested include caspase-1 p10 (C-20): (A-E) and caspase-1 (A-19): (F-J).| Product Name | caspase-1 Antibody (A-19) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping at the N-terminus of caspase-1 of human origin |
| Modification Notes | Caspase-1, originally designated ICE (for IL-1 converting enzyme), is a member of the group of caspases with large prodomains. Caspase-1 promotes maturation of interleukin IL-1∫ and interleukin18 (IL-18) by proteolytic cleavage of precursor forms into biologically active pro-inflamatory cytokines. Active caspase-1, a (p20/p10)2 tetramer, is necessary and sufficient for cleavage of precursor IL-1 as well as for induction of apoptosis in some cell lines. The highly conserved family of caspases mediate many of the morphological and biochemical features of apoptosis, including structural dismantling of cell bodies and nuclei, fragmentation of genomic DNA, destruction of regulatory proteins and propagation of other pro-apoptotic molecules. The human Caspase-1 gene maps to chromosome 2q14 and encodes a cytoplasmic protein expressed in liver, heart, skeletal muscle kidney and testis. Caspase-1 has been implicated in inflammation, septic shock, and other situations such as wound healing and the growth of certain leukemias. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFFCELISA |
| recommended for detection of the caspase-1 precursor, CARD 17, and COP of human origin by WB, IP, IF, FCM and ELISA non cross-reactive with caspase-1 p10 or caspase-1 p20: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping at the N-terminus of caspase-1 of human origin |
|---|---|
| Tissue Specificity | epitope mapping at the N-terminus of caspase-1 of human origin |
| Entrez Gene | 834 |
|---|
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.
Application
Intracellular FCM analysis of fixed and permeabilized Jurkat cells. Black line histogram represents the isotype control, normal rabbit IgG: .
Application
Antibodies tested include caspase-1 p10 (C-20): (A-E) and caspase-1 (A-19): (F-J).| Application Notes | recommended for detection of the caspase-1 precursor, CARD 17, and COP of human origin by WB, IP, IF, FCM and ELISA non cross-reactive with caspase-1 p10 or caspase-1 p20: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
