Application
Western blot analysis of Pim-3 expression in LNCaP (A) and Jurkat (B) whole cell lysates.Application
Western blot analysis of Pim-3 expression in LNCaP whole cell lysate.| Product Name | Pim-3 Antibody (H-40) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 51-90 mapping near the N-terminus of Pim-3 of human origin |
| Modification Notes | The Pim (provirus integration site for Moloney murine leukemia virus) family serine/threonine protein kinases were first identified in studies examining genes targeted for proviral insertion in murine leukemia virus-induced T lymphomas. Increased levels of Pim kinases predispose cells to lymphomagenesis and enhance the activity of mitogenic proteins such as p100, c-Myb, and Cdc25A. In addition, Pim kinases are also involved in modulation of synaptic strength in neurons and anti-apoptotic signaling in hematopoietic progenitor cells. Pim-3, a member of the proto-oncogene Pim family that expresses serine/threonine kinase activity, shares significant homology with Pim-1 serine/threonine protein kinases. Pim-3 may function as a mediator of synaptic plasticity in the brain and is presumably involved in the anti-apoptosis process and cell cycle progression as well as the proliferation of human hepatoma cell lines. The Pim-3 protein is widely expressed, however no expression is observed in the colon, thymus, or small intestine. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Pim-3 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 51-90 mapping near the N-terminus of Pim-3 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 51-90 mapping near the N-terminus of Pim-3 of human origin |
| Entrez Gene | 415116 |
|---|
Application
Western blot analysis of Pim-3 expression in LNCaP (A) and Jurkat (B) whole cell lysates.
Application
Western blot analysis of Pim-3 expression in LNCaP whole cell lysate.| Application Notes | recommended for detection of Pim-3 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
