Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human liver tissue showing membrane and cytoplasmic staining of hepatocytes.| Product Name | ASGPR1/2 Antibody (FL-291) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-291 representing full length ASGPR1 of human origin |
| Modification Notes | The Asialoglycoprotein Receptor (ASGPR, also designated hepatic lectin) is a type II integral membrane protein and is expressed in hepatic cells. ASGPR is composed of two homologous subunits, ASGPR1 and ASGPR2, that form multimeric complexes. Both ASGPR1 and ASGPR2 contain four functional domains, which include a cytosolic domain, a transmembrane domain, a stalk domain and a carbohydrate recognition domain (CRD). The CRD allows ASGPR to bind glycoproteins with terminal galactose and N-acetylgalactosamine residues while in the presence of calcium. After binding, the ASGPR-glycoprotein complex is then internalized into the cell, where the receptor and ligand are dissociated and ASGPR returns to the cell membrane. ASGPR can also bind hepatitis B virus (HBV) and mediate the HBV-infection of liver cells. The specific interaction with HBV makes ASGPR a potential target for therapeutic purposes. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of ASGPR1 and 2 and MASGP-BP of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-291 representing full length ASGPR1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-291 representing full length ASGPR1 of human origin |
| Entrez Gene | 432 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human liver tissue showing membrane and cytoplasmic staining of hepatocytes.| Application Notes | recommended for detection of ASGPR1 and 2 and MASGP-BP of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
