Application
Western blot analysis of CD2 expression in non-transfected 293T: (A), human CD2 transfected 293T: (B) and Jurkat (C) whole cell lysates.Application
Western blot analysis of CD2 expression in non-transfected 293T: (A), human CD2 transfected 293T: (B) and Jurkat (C) whole cell lysates.Application
Western blot analysis of CD2 expression in Jurkat whole cell lysate.| Product Name | CD2 Antibody (H-230) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 52-281 mapping within an internal region of CD2 of human origin |
| Modification Notes | CD2 (also designated E-rosette receptor) interacts through its amino-terminal domain with the extracellular domain of CD58 (also designated CD2 ligand) to mediate cell adhesion. CD2/CD58 binding can enhance antigen-specific T cell activation. CD2 is a transmembrane glycoprotein that is expressed on peripheral blood T lymphocytes, NK cells and thymocytes, as well as on mouse B cells and rat splenic macrophages. CD58 is a heavily glycosylated protein with a broad tissue distribution in hematopoietic and other cells, including endothelium. Interaction between CD2 and its counterreceptor LFA3 (CD58) on opposing cells optimizes immune system recognition, thereby facilitating communication between helper T lymphocytes and antigen-presenting cells, as well as between cytolytic effectors and target cells. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of CD2 of human origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 52-281 mapping within an internal region of CD2 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 52-281 mapping within an internal region of CD2 of human origin |
| Entrez Gene | 914 |
|---|
Application
Western blot analysis of CD2 expression in non-transfected 293T: (A), human CD2 transfected 293T: (B) and Jurkat (C) whole cell lysates.
Application
Western blot analysis of CD2 expression in non-transfected 293T: (A), human CD2 transfected 293T: (B) and Jurkat (C) whole cell lysates.
Application
Western blot analysis of CD2 expression in Jurkat whole cell lysate.| Application Notes | recommended for detection of CD2 of human origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
