Application
Western blot analysis of POSH expression in KNRK (A) and HeLa (B) whole cell lysates.| Product Name | POSH Antibody (M-290) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 591-880 mapping near the C-terminus of POSH of mouse origin |
| Modification Notes | Rho, Rac and Cdc42 are members of the small GTPase family. These proteins act as molecular switches, cycling between an active GTP-bound state and an inactive GDP-bound state (1). Activation of these proteins results in rearrangements of filamentous actin and the formation of actin stress fibers (2,3). Many of the targets of these GTPases are involved in signal transduction events mediated by Src3 homology (SH3) domains (4,5). POSH, for “plenty of SH3s”, is a Rac binding protein with four SH3 domains. POSH preferentially interacts with the GTP form of RAC and not with the GDP-bound RAC. Ectopic expression of POSH elicits JNK activation and nuclear translocation of NFkB, suggesting that POSH is involved in Rac regulation of these kinase pathways. Overexpression of POSH has also been shown to induce apoptosis (6). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of POSH of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 591-880 mapping near the C-terminus of POSH of mouse origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 591-880 mapping near the C-terminus of POSH of mouse origin |
| Entrez Gene | 57630 |
|---|
Application
Western blot analysis of POSH expression in KNRK (A) and HeLa (B) whole cell lysates.| Application Notes | recommended for detection of POSH of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
