Application
Western blot analysis of SPT16 expression in HeLa (A), A549 (B), SH-SY5Y (C), K-562 (D) and Jurkat (E) nuclear extracts.Application
Western blot analysis of SPT16 expression in non-transfected: (A) and mouse SPT16 transfected: (B) 293T whole cell lysates and HeLa nuclear extract (C).Application
Immunofluorescence staining of methanol-fixed UV-treated HeLa cells showing nuclear localization.Application
Western blot analysis of SPT16 expression in UV-treated HeLa whole cell lysate.| Product Name | SPT16 Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 748-1047 mapping at the C-terminus of SPT16 of human origin |
| Modification Notes | Expression of protein-coding genes requires the association of specific transcription factors, RNA polymerase and various accessory factors. These accessory factors are distinguished as either histone acetyltransferases or ATP-dependent chromatin-remodeling enzymes, which include FACT (for facilitates chromatin transcription), and they facilitate transcription initiation on DNA packaged into chromatin. FACT is a chromatin-specific elongation factor required for transcription of chromatin templates, and it specifically interacts with nucleosomes and histone H2A/H2B dimers, to promote nucleosome disassembly upon transcription. FACT represents a complex between SPT16, a homologue of the Saccharomyces cerevisiae Spt16/Cdc68 protein, and the high-mobility group (HMG)-1-like protein structure-specific recognition protein-1 (SSRP-1) (5,6). Similar to other (HMG) domain containing proteins, which are characterized by their ability to bend target DNAs, SSRP1 and the murine ortholog T160, physically interact with serum response factors (SRF) and function as a positive co regulatory proteins involved in modulating SRF-dependent gene expression. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of SPT16 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 748-1047 mapping at the C-terminus of SPT16 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 748-1047 mapping at the C-terminus of SPT16 of human origin |
| Entrez Gene | 11198 |
|---|
Application
Western blot analysis of SPT16 expression in HeLa (A), A549 (B), SH-SY5Y (C), K-562 (D) and Jurkat (E) nuclear extracts.
Application
Western blot analysis of SPT16 expression in non-transfected: (A) and mouse SPT16 transfected: (B) 293T whole cell lysates and HeLa nuclear extract (C).
Application
Immunofluorescence staining of methanol-fixed UV-treated HeLa cells showing nuclear localization.
Application
Western blot analysis of SPT16 expression in UV-treated HeLa whole cell lysate.| Application Notes | recommended for detection of SPT16 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
