Application
Immunofluorescence staining of methanol-fixed HeLa cells showing membrane localization.| Product Name | syncytin Antibody (H-280) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 33-312 mapping near the N-terminus of syncytin of human origin |
| Modification Notes | Syncytin, also known as ERVWE1 (endogenous retroviral family W, env(C7), member 1) is a human endogenous retrovirus family W (HERV-W) envelope protein that is expressed in placental syncytiotrophoblast and is involved in fusion of the cytotrophoblast cells to form the multinucleated syncytial layer of the placenta. Syncytin is an envelope protein after in vitro transcription-translation and undergoes glycosylation. It is predicted to undergo post-translational cleavage into a surface (SU) subunit and a transmembrane (TM) subunit. Syncytin is similar to other retroviral envelope proteins in possessing a furin cleavage site that separates the surface (SU) and transmembrane (TM) proteins, which form a heterodimer. Northern blot analysis has been shown to detect 8.0-, 3.1-, and 1.3-kb HERV-W transcripts only in placenta. Syncytin expression can increase three-fold in astrocytes and glial cells within acute demyelinating lesions of patients with multiple sclerosis compared to controls. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of syncytin surface protein subunit of human origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 33-312 mapping near the N-terminus of syncytin of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 33-312 mapping near the N-terminus of syncytin of human origin |
| Entrez Gene | 30816 |
|---|
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing membrane localization.| Application Notes | recommended for detection of syncytin surface protein subunit of human origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
