Application
Western blot analysis of caspase-4 expression in non-transfected: (A) and human caspase-4 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of caspase-4 and caspase-5 precursor expression in Jurkat whole cell lysate.| Product Name | caspase-4/5 p10 Antibody (H-60) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 341-400 of caspase-5 of human origin |
| Modification Notes | Caspases are cysteine proteases which play important roles in the activation of cytokines and in apoptosis. The ICE subfamily of caspases comprises peptides closely related to caspase-1, which promotes maturation of interleukin 1∫ (IL-1∫) and interleukin-18 (IL-18) by proteolytic cleavage of precursor forms to generate biologically active peptides. Both caspase-4 and caspase-5 are members of the caspase-1 subfamily, and are more closely related to each other than to other homologs. Caspase-5 (also designated ICErel-III, TY, ICH-3 and caspase-12 in mouse), can cleave its own precursor, an activity that requires the Cysteine 245 residue. The human caspase-5 gene maps to chromosome 11q22.2-q22.3 and encodes a protein whose expression is barely detectable in most tissues except brain. Caspase-4 cleaves caspase-1 and has two subunits derived from the precursor sequence by an autocatalytic mechanism or by cleavage by caspase-8. Caspase-4 is widely expressed, with highest levels in spleen and lung, and is not found in the brain. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of caspase-4 and caspase-5 p10 subunit of human origin and, to a lesser extent, caspase-1 and caspase-12 of mouse, rat and human origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 341-400 of caspase-5 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 341-400 of caspase-5 of human origin |
| Entrez Gene | 837 |
|---|
Application
Western blot analysis of caspase-4 expression in non-transfected: (A) and human caspase-4 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of caspase-4 and caspase-5 precursor expression in Jurkat whole cell lysate.| Application Notes | recommended for detection of caspase-4 and caspase-5 p10 subunit of human origin and, to a lesser extent, caspase-1 and caspase-12 of mouse, rat and human origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
