Application
Western blot analysis of MIP-1α expression in non-transfected: (A) and human MIP-1α transfected: (B) 293T whole cell lysates.| Product Name | MIP-1α Antibody (FL-92) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-92 representing full length MIP-1α of human origin |
| Modification Notes | Chemokines are members of a superfamily of small inducible, secreted, pro-inflammatory cytokines. Members of the chemokine family exhibit 20 to 50% homology in their predicted amino acid sequences and are divided into four subfamilies. In C-C (or b) subfamily, the first two cysteines are adjacent. C-C chemokines are chemoattractants and activators for monocytes and T cells. C-C subfamily members include macrophage inflammatory protein (MIP)-1å, MIP-1∫, MIP-2, MIP-3å, MIP-3∫, MIP-4, HCC-1, MIP-5 (or HCC-2), RANTES, MCP-1/2/3 (and the murine homologs JE and MARC), I-309, murine C10 and TCA3. Research has shown that MIP-1∫ is more selective than MIP-1å, primarily attracting CD4+ T lymphocytes, with a preference for T cells of the naive phenotype. MIP-1å is a more potent lymphocyte chemoattractant than MIP-1∫ and exhibits a broader range of chemoattractant specificities. It has been suggested that CD8+ T lymphocytes are involved in the control of HIV infection in vivo by the release of HIV-suppressive factors (HIV-SF). MIP-1å has been identified as one of the major HIV-SFs produced by CD8+ T cells, along with MIP-1∫ and RANTES. Recombinant human MIP-1å acts as an inhibitor of different strains of HIV-1, HIV-2 and SIV infection in a dose-dependent manner. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of MIP-1α and LD78β and, to a lesser extent, MIP-1β and MIP-4 of mouse, rat and human origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-92 representing full length MIP-1&alpha of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-92 representing full length MIP-1α of human origin |
| Entrez Gene | 6348 |
|---|
Application
Western blot analysis of MIP-1α expression in non-transfected: (A) and human MIP-1α transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of MIP-1α and LD78β and, to a lesser extent, MIP-1β and MIP-4 of mouse, rat and human origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
