Application
Western blot analysis of DAP-5 expression in HeLa nuclear extract.Application
Western blot analysis of DAP-5 expression in HeLa (A) and MCF7 (B) nuclear extracts.Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.| Product Name | DAP-5 Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 608-907 mapping at the C-terminus of DAP-5 of human origin |
| Modification Notes | Death-associated protein 5 (DAP-5) (also known as p97 and NAT1) is a member of the eukaryotic translation initiation factor-4G (elF4G) family. DAP-5 is ubiquitously expressed and is highly conserved among species. In response to activated FAS or p53, caspase cleaves DAP-5 at position 790 to yield a C-terminal truncated protein, which is capable of forming complexes with eIF4A and eIF3. DAP-5 has homology to the carboxy-terminal portion of eIF4G, but lacks the N-terminal region of eIF4G, which is responsible for association with the CAP binding protein eIF4E. By forming translationally inactive complexes with eIF4A and eIF3, but not with eIF4E, DAP-5 functions as a general repressor of translation. During apotosis, the caspase-activated DAP-5 can mediate CAP-independent translation at least from its own internal ribosome entry site, thus resulting in a positive feedback loop responsible for the continuous translation of DAP-5. DAP-5 is also required for cellular differentiation, as it controls specific gene expression pathways. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of DAP-5 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 608-907 mapping at the C-terminus of DAP-5 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 608-907 mapping at the C-terminus of DAP-5 of human origin |
| Entrez Gene | 1982 |
|---|
Application
Western blot analysis of DAP-5 expression in HeLa nuclear extract.
Application
Western blot analysis of DAP-5 expression in HeLa (A) and MCF7 (B) nuclear extracts.
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.| Application Notes | recommended for detection of DAP-5 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
