Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human oral mucosa tissue showing nuclear and cytoplasmic staining of squamous epithelial cells.Application
Western blot analysis of Pygopus 2 expression in non-transfected: (A) and mouse Pygopus 2 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of Pygopus 2 expression in HeLa whole cell lysate.| Product Name | Pygopus 2 Antibody (H-216) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 127-339 mapping within an internal region of Pygopus 2 of human origin |
| Modification Notes | Pygopus 2, also known as PYGO2, is a 406 amino acid protein that is the human homolog of the Drosophila pygopus protein. Localized to the nucleus, Pygopus 2 contains one PHD finger that interacts with the homology domain of the Wnt signaling protein Bcl-9. This interaction joins Pygopus 2 with the ∫-catenin/TCF complex (a crucial complex in Wnt signaling), thereby allowing ∫-catenin to transcriptionally activate Wnt target genes. Association of Pygopus 2 with proteins involved in the Wnt signaling pathway is thought to regulate proper signal transduction, as absence of the Pygopus 2/∫-catenin interaction may play a role in development of B-cell malignancies. In addition, Pygopus 2 expression is upregulated in and required for the growth of breast cancer cells, suggesting a crucial role in carcinogenesis. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Pygopus 2 of mouse, rat and human origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 127-339 mapping within an internal region of Pygopus 2 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 127-339 mapping within an internal region of Pygopus 2 of human origin |
| Entrez Gene | 90780 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human oral mucosa tissue showing nuclear and cytoplasmic staining of squamous epithelial cells.
Application
Western blot analysis of Pygopus 2 expression in non-transfected: (A) and mouse Pygopus 2 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of Pygopus 2 expression in HeLa whole cell lysate.| Application Notes | recommended for detection of Pygopus 2 of mouse, rat and human origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
