Application
Antibodies tested include p-AMPKα1/2 (Thr 183/172): (A, B) and AMPKα1/2 (H-300): (C, D).Application
Antibodies tested include p-AMPKα1/2 (Thr 183/172): (A,C) and AMPKα1 (71.54): (B,D).Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human lung carcinoma tissue showing cytoplasmic localization.| Product Name | p-AMPKα1/2 Antibody (Thr 183/172) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to phosphorylated Thr 172 of AMPKα1 of human origin |
| Modification | Phosphoralated |
| Modification Notes | Five-prime-AMP-activated protein kinase, known as AMPK, is a heterotrimeric complex that comprises of a catalytic å subunit, and regulatory ∫ and ©. AMPK protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. AMPK is activated by high AMP and low ATP via a mechanism involving allosteric regulation, promotion of phosphorylation by an upstream protein kinase known as AMPK kinase (AMPKK), and inhibition of dephosphorylation. Activated AMPK can phosphorylate and regulate in vivo hydroxymethylglutaryl-CoA reductase and acetyl-CoA carboxylase, which are key regulatory enzymes of sterol synthesis and fatty acid synthesis, respectively. The major regulatory site phosphorylated by AMPKK on AMPKå has been identified as Thr 172 within the activation loop between the DFG and APE motifs of the å subunits. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHC |
| recommended for detection of Thr 183 phosphorylated AMPK α1 and Thr 172 phosphorylated AMPK α2 isoforms of the catalytic subunit of mouse, rat and human origin by WB, IP, IF and IHC(P): |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to phosphorylated Thr 172 of AMPK&alpha 1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to phosphorylated Thr 172 of AMPKα1 of human origin |
| Entrez Gene | 5562 |
|---|
Application
Antibodies tested include p-AMPKα1/2 (Thr 183/172): (A, B) and AMPKα1/2 (H-300): (C, D).
Application
Antibodies tested include p-AMPKα1/2 (Thr 183/172): (A,C) and AMPKα1 (71.54): (B,D).
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human lung carcinoma tissue showing cytoplasmic localization.| Application Notes | recommended for detection of Thr 183 phosphorylated AMPK α1 and Thr 172 phosphorylated AMPK α2 isoforms of the catalytic subunit of mouse, rat and human origin by WB, IP, IF and IHC(P): |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
