| Product Name | Sarcospan Antibody (H-50) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 119-168 mapping within an extracellular domain of Sarcospan of human origin |
| Modification Notes | The dystrophin-glycoprotein complex (DGC) is a multisubunit protein complex that spans the sarcolemma and forms a link between the subsarcolemmal cytoskeleton and the extracellular matrix. Defects in components of the DGC cause muscular dystrophy, indicating that the DGC plays important roles in muscular function and viability. Sarcospan (also designated K-Ras oncogene-associated protein and Kirstein-Ras-associated protein), a member of this complex, contains four transmembrane spanning helices with intracellular N- and C-terminal domains. The expression of Sarcospan is reduced in muscle from patients with Duchenne muscular dystrophy. Sarcospan mRNAs are expressed in a range of cell lines, tumors, and normal tissue, with very high expression levels in muscle. Two isoforms of Sarcospan, SPN1 and SPN2, are produced by alternative splicing. SPN1 is expressed in heart and skeletal muscle, whereas SPN2 is expressed in heart, skeletal muscle, thymus, pros-tate, testis, ovary, small intestine, colon and spleen. The sarcoglycan complex in striated muscle is a heterotetrameric unit integrally associated with Sarcospan in the dystrophin-glycoprotein complex and it is also linked to the signaling protein, neural nitric oxide synthase, through a-Syntrophin that associated with dystrobrevin. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Sarcospan of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine and bovine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 119-168 mapping within an extracellular domain of Sarcospan of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 119-168 mapping within an extracellular domain of Sarcospan of human origin |
| Entrez Gene | 8082 |
|---|
| Application Notes | recommended for detection of Sarcospan of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine and bovine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
