Application
Western blot analysis of apoE expression in non-transfected: (A) and mouse apoE transfected: (B) 293T whole cell lysates.Application
Western blot analysis of apoE expression in K-562 (A) and human platelet (B) whole cell lysates.Application
Western blot analysis of apoE expression in SK-N-MC (A) and Caki-1 (B) whole cell lysates.Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.| Product Name | apoE Antibody (M-293) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 19-311 mapping at the C-terminus of apoE of mouse origin |
| Modification Notes | Apolipoprotein-E (apoE) is a protein component of plasma lipoproteins that mediates the binding, internalization and catabolism of lipoprotein particles. It can serve as a ligand for several lipoprotein receptors, including the LDL (ApoB/E) receptor and the hepatic apoE (chylomicron remnant) receptor. apoE is produced in most organs and occurs in all plasma lipoprotein fractions, constituting 10-20% of VLDL (very low density lipoprotein) and 1-2% of HDL (high density lipoprotein). Three major isoforms of apoE have been described in human (E2, E3 and E4) which differ by only one or two amino acids. Estrogen receptor has been shown to upregulate apoE gene expression via the ERa-mediated pathway, indicating a potential role for apoE in atherosclerosis. This is consistent with studies in mice in which plasma apoE levels were raised, thereby protecting the mice from diet-induced atherosclerosis. apoE has also been shown to be a potent inhibitor of proliferation and thus may play a role in angiogenesis, tumor cell growth and metastasis. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of apoE of mouse, rat and, to a lesser extent, human origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 19-311 mapping at the C-terminus of apoE of mouse origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 19-311 mapping at the C-terminus of apoE of mouse origin |
Application
Western blot analysis of apoE expression in non-transfected: (A) and mouse apoE transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of apoE expression in K-562 (A) and human platelet (B) whole cell lysates.
Application
Western blot analysis of apoE expression in SK-N-MC (A) and Caki-1 (B) whole cell lysates.
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.| Application Notes | recommended for detection of apoE of mouse, rat and, to a lesser extent, human origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
