Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human small intestine tissue showing nuclear and apical membrane staining of glandular cells.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human tonsil tissue showing nuclear staining of lymphoid cells and squamous epithelial cells (low and high magnification). Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Immunofluorescence staining of normal mouse liver frozen section showing nuclear staining.| Product Name | TRAP150 Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 191-490 mapping within an internal region of TRAP150 of human origin |
| Modification Notes | In mammalian cells, transcription is regulated in part by high molecular weight coactivating complexes that mediate signaling between transcriptional activators and initiation factors. These complexes include the thyroid hormone receptor-associated protein (TRAP) complex, which interacts with thyroid receptors (TR), vitamin D receptors and other steroid receptors to facilitate hormone induced transcriptional activation. The TRAP complex consists of numerous proteins ranging in size including TRAP95, TRAP100, TRAP150, TRAP220 and TRAP230, that are characterized by the presence of a nuclear receptor recognition motif which mediates the ligand-dependent binding of TRAP proteins to the nuclear receptors. TRAP220 and TRAP100 are widely expressed and most abundantly detected in skeletal muscle, heart and placenta. TRAP95, TRAP150 and TRAP230 facilitate TR induced transcription by associating with an additional transcriptional coactivating complex SMCC (SRB and MED protein cofactor complex), which consists of various subunits that share homology with several components of the yeast transcriptional mediator complexes. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISAGSChIP |
| recommended for detection of TRAP150 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 191-490 mapping within an internal region of TRAP150 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 191-490 mapping within an internal region of TRAP150 of human origin |
| Entrez Gene | 9967 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human small intestine tissue showing nuclear and apical membrane staining of glandular cells.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human tonsil tissue showing nuclear staining of lymphoid cells and squamous epithelial cells (low and high magnification). Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Immunofluorescence staining of normal mouse liver frozen section showing nuclear staining.| Application Notes | recommended for detection of TRAP150 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
