Application
Western blot analysis of WAVE expression in non-transfected: (A) and mouse WAVE transfected: (B) 293T whole cell lysates and mouse brain tissue extract (C).Application
Western blot analysis of WAVE3 expression in SK-N-MC (A) and IMR-32 (B) whole cell lysates.Application
Western blot analysis of WAVE3 expression in MOLT-4 whole cell lysate.| Product Name | WAVE Antibody (H-180) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-180 of WAVE1 of human origin |
| Modification Notes | WASP (for Wiskott-Aldrich syndrome protein) and N-WASP are downstream effectors of Cdc42 that are implicated in Actin polymerization and cytoskeletal organization. The WASP family also includes VASP (vasodilator-stimulated phosphoprotein) and Mena (for mammalian enabled protein), which accumulate at focal adhesions and are also involved in the regulation of the Actin cytoskeleton. The WAVE proteins are related to the WASP family proteins and are likewise involved in mediating Actin reorganization downstream of the Rho family of small GTPases. The protein homologs WAVE1 and WAVE2 regulate membrane ruffling by inducing the formation of Actin filament clusters in response to GTP binding and by activating Rac. They mediate Actin polymerization by cooperating with the Arp2/3 complex, thereby promoting the formation of Actin filaments. WAVE1, which is also designated SCAR (suppressor of cAR), is expressed primarily in the brain, while WAVE2 is widely expressed, with the expression highest in peripheral blood leukocytes. WAVE3 forms a multiprotein complex that links receptor kinases with Actin and plays a role in the transduction of signals involving changes in cell shape, function or motility. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of WAVE1, WAVE2 and WAVE3 of mouse, rat and human origin and WAVE4 of human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-180 of WAVE1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-180 of WAVE1 of human origin |
| Entrez Gene | 8936 |
|---|
Application
Western blot analysis of WAVE expression in non-transfected: (A) and mouse WAVE transfected: (B) 293T whole cell lysates and mouse brain tissue extract (C).
Application
Western blot analysis of WAVE3 expression in SK-N-MC (A) and IMR-32 (B) whole cell lysates.
Application
Western blot analysis of WAVE3 expression in MOLT-4 whole cell lysate.| Application Notes | recommended for detection of WAVE1, WAVE2 and WAVE3 of mouse, rat and human origin and WAVE4 of human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
