Application
Western blot analysis of Gα s expression in Jurkat (A) and K-562 (B) whole cell lysates.Application
Western blot analysis of Gα s expression in TT whole cell lysate.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human malignant glioma tissue showing cytoplasmic and membrane staining of tumor cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Western blot analysis of rat recombinant Gβ s fusion protein.| Product Name | Gα s Antibody (K-20) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping within the N-terminus of Gα s of human origin |
| Modification Notes | Heterotrimeric G proteins function to relay information from cell surface receptors to intracellular effectors (1). Each of a very broad range of receptors specifically detects an extracellular stimulus (a photon, pheromone, odorant, hormone or neurotransmitter) while the effectors (e.g., adenylyl cyclase), which act to generate one or more intracellular messengers, are less numerous. In mammals, G protein å, ∫ and © polypeptides are encoded by at least 16, 4 and 7 genes, respectively (2-5). Most interest in G proteins has been focused on their a subunits, since these proteins bind and hydrolyze GTP and most obviously regulate the activity of the best studied effectors. Evidence, however, has established an important regulatory role for the ∫© subunits (6-8). The Gs subfamily of Gå subunits includes two closely related proteins, Gå s and Gå olf, which respectively stimulate adenylyl cyclase and mediate response to olfactory stimuli (9). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of Gα s of mouse, rat, human and bovine origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping within the N-terminus of G&alpha s of human origin |
|---|---|
| Tissue Specificity | epitope mapping within the N-terminus of Gα s of human origin |
| Entrez Gene | 2778 |
|---|
Application
Western blot analysis of Gα s expression in Jurkat (A) and K-562 (B) whole cell lysates.
Application
Western blot analysis of Gα s expression in TT whole cell lysate.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human malignant glioma tissue showing cytoplasmic and membrane staining of tumor cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Western blot analysis of rat recombinant Gβ s fusion protein.| Application Notes | recommended for detection of Gα s of mouse, rat, human and bovine origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
