Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization.Application
Antibodies tested include CBP (A-22): , p300 (N-15): , PCAF (H-369): , and Brg-1 (H-88): . CD3ε and Oct-2 promoter regions were employed as positive and negative controls, respectively. DNA was isolated from Rag-/- thymocytes or Rag-/- thymocytes expressing TCRβ. Data kindly provided by J. Imbert and reproduced from Yeh, J-H., et al., Nucleic Acids Research, 2002, 30: 1944-1951, with permission from Oxford University Press.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human lung tumor showing nuclear staining.Application
Western blot analysis of Brg-1 expression in HeLa (A) and K-562 (B) nuclear extracts.| Product Name | Brg-1 Antibody (H-88) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 209-296 mapping near the N-terminus of Brg-1 of human origin |
| Modification Notes | The SWI-SNF complex is involved in the activation of transcription via the remodeling of nucleosome structure in an ATP-dependent manner. Brm (also designated SNF2å) and Brg-1 (also designated SNF2ß) are the ATPase subunits of the mammalian SWI-SNF complex. Brm, Brg-1, Ini1 (integrase interactor 1, also designated SNF5), BAF155 (also designated SRG3) and BAF170 are thought to comprise the functional core of the SWI-SNF complex. Addition of Ini1, BAF155 and BAF170 to Brg-1 appears to increase remodeling activity. Other complex subunits are thought to play regulatory roles. hSNF2L and hSNF2H both appear to be homologs of Drosophila ISWI, a Brm related ATPase that is present in chromatin remodeling complexes other than SWI/SNF, including the NURF (nucleosome remodeling factor). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISAGSChIP |
| recommended for detection of Brg-1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 209-296 mapping near the N-terminus of Brg-1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 209-296 mapping near the N-terminus of Brg-1 of human origin |
| Entrez Gene | 6597 |
|---|
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization.
Application
Antibodies tested include CBP (A-22): , p300 (N-15): , PCAF (H-369): , and Brg-1 (H-88): . CD3ε and Oct-2 promoter regions were employed as positive and negative controls, respectively. DNA was isolated from Rag-/- thymocytes or Rag-/- thymocytes expressing TCRβ. Data kindly provided by J. Imbert and reproduced from Yeh, J-H., et al., Nucleic Acids Research, 2002, 30: 1944-1951, with permission from Oxford University Press.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human lung tumor showing nuclear staining.
Application
Western blot analysis of Brg-1 expression in HeLa (A) and K-562 (B) nuclear extracts.| Application Notes | recommended for detection of Brg-1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
