Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human placenta tissue showing nuclear staining of trophoblastic cells.Application
Immunofluorescence staining of normal mouse lymph node frozen section showing nuclear staining.| Product Name | NRF-1 Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 204-503 mapping at the C-terminus of NRF-1 of human origin |
| Modification Notes | Nuclear respiratory factor-1 (NRF-1) is a transcriptional activator that has been implicated in the nuclear control of respiratory chain expression in mammalian cells. The NRF-1 gene is expressed during oogenesis and during the early stages of embryogenesis. In vitro studies have implicated NRF-1 in the transcriptional expression of nuclear genes required for mitochondrial respiratory function, as well as for other fundamental cellular activities. While most isolated wild-type and NRF-1+/- blastocysts continue to develop normally in vitro, NRF-1-/- blastocysts lack this ability, despite their normal morphology. NRF-1 is specifically required in the maintenance of mtDNA and respiratory chain function during early embryogenesis. NRF-1 also plays a key role in cellular adaptation to energy demands by translating physiological signals into an increased capacity for generating energy. Additionally, NRF-1 is a major transcription factor that binds the promoter in brain and testis. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISAGSChIP |
| recommended for detection of NRF-1 long and short isoforms of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 204-503 mapping at the C-terminus of NRF-1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 204-503 mapping at the C-terminus of NRF-1 of human origin |
| Entrez Gene | 4899 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human placenta tissue showing nuclear staining of trophoblastic cells.
Application
Immunofluorescence staining of normal mouse lymph node frozen section showing nuclear staining.| Application Notes | recommended for detection of NRF-1 long and short isoforms of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
