Application
Western blot analysis of twist expression in HeLa nuclear extract (A) and WI 38 (B), AtT-20/D16vF2 (C), JAR (D), MES-SA/Dx5 (E) and JEG-3 (F) whole cell lysatesApplication
Western blot analysis of human recombinant twist fusion protein.| Product Name | twist Antibody (H-81) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 121-202 mapping at the C-terminus of twist of human origin |
| Modification Notes | Members of the myogenic determination family are basic helix-loop-helix (bHLH) proteins that can be separated into two classes. Class A proteins include the ubiquitously expressed E-box binding factors E12/E47, ITF2 and HEB (BETA1 or HTF4). Class B proteins such as MyoD, myogenin and NeuroD (BETA2) are transiently expressed and exhibit a much more limited tissue distribution. Class A proteins heterodimerize with class B proteins to activate DNA transcription. Working in opposition to these positively acting factors are a specialized group of proteins that function as dominant negative regulators. Muscle tissue is derived from a subset of cells originating from the embryonic mesoderm. The novel basic helix-loop-helix (bHLH) transcription factor twist is a putative regulator of mesodermal differentiation and myogenesis. Twist is expressed throughout the epithelial somite but not in the myotome. Twist requires dimerization with the E proteins and inhibits myogenic regulatory factors. It has been implicated as regulator of the temporal and spatial formation of myotomes. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISAGSChIP |
| recommended for detection of twist and twist2 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 121-202 mapping at the C-terminus of twist of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 121-202 mapping at the C-terminus of twist of human origin |
| Entrez Gene | 7291 |
|---|
Application
Western blot analysis of twist expression in HeLa nuclear extract (A) and WI 38 (B), AtT-20/D16vF2 (C), JAR (D), MES-SA/Dx5 (E) and JEG-3 (F) whole cell lysates
Application
Western blot analysis of human recombinant twist fusion protein.| Application Notes | recommended for detection of twist and twist2 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
