Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human pancreas tissue showing cytoplasmic staining of exocrine glandular cells and Islets of Langerhans.Application
Western blot analysis of BAP31 expression in mouse pancreas tissue extract.Application
Western blot analysis of BAP31 expression in non-transfected: (A) and mouse BAP31 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of BAP31 expression in non-transfected: (A) and human BAP31 transfected: (B) 293T whole cell lysates.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human pancreas tissue showing cytoplasmic staining of exocrine glandular cells and islet cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.Application
Immunofluorescence staining of normal mouse intestine frozen section showing cytoplasmic and membrane staining.Application
Western blot analysis of BAP31 expression in BJAB (A) and HL-60 (B) whole cell lysates.| Product Name | BAP31 Antibody (H-90) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 157-246 mapping within a C-terminal cytoplasmic domain of BAP31 of human origin |
| Modification Notes | BAP31, a human Bcl-2-interacting protein, is an integral membrane protein that is a component of a protein complex in the endoplasmic reticulum. This protein complex mechanically bridges an apoptosis-initiating caspase, like procaspase-8, with the anti-apoptotic regulator Bcl-2 or Bcl-XL. The cytosolic domain of BAP31 contains two identical caspase recognition sites, which are preferentially cleaved by initiator caspases, including caspase 8. Cleavage of BAP31 during apoptosis generates a p20 fragment, which remains integrated in the membrane and, when expressed ectopically, is a potent inducer of cell death. BAP31 cleavage is important for manifesting cytoplasmic apoptotic events associated with membrane fragmentation and in the cross talk between mitochondria and the endoplasmic reticulum during Fas- mediated apoptosis. The BAP31 gene is ubiquitously expressed in murine tissues and is located on the X chromosome in both mouse and human. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of BAP31 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 157-246 mapping within a C-terminal cytoplasmic domain of BAP31 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 157-246 mapping within a C-terminal cytoplasmic domain of BAP31 of human origin |
| Entrez Gene | 10134 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human pancreas tissue showing cytoplasmic staining of exocrine glandular cells and Islets of Langerhans.
Application
Western blot analysis of BAP31 expression in mouse pancreas tissue extract.
Application
Western blot analysis of BAP31 expression in non-transfected: (A) and mouse BAP31 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of BAP31 expression in non-transfected: (A) and human BAP31 transfected: (B) 293T whole cell lysates.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human pancreas tissue showing cytoplasmic staining of exocrine glandular cells and islet cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.
Application
Immunofluorescence staining of normal mouse intestine frozen section showing cytoplasmic and membrane staining.
Application
Western blot analysis of BAP31 expression in BJAB (A) and HL-60 (B) whole cell lysates.| Application Notes | recommended for detection of BAP31 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
