Application
Western blot analysis of Barhl2 expression in Hep G2 whole cell lysate.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human lung tissue showing nuclear localization.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human kidney tissue showing nuclear and cytoplasmic localization.| Product Name | Barhl2 Antibody (L-23) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of Barhl2 of h and r origin by WB, IP, IF, IHC(P) and ELISA |
| Modification Notes | BarH2 is a homeobox containing protein required for the fate determination of external sensory organs in Drosophila. Barhl2 (BarH-like 2 homeobox protein), also known as MBH1 in rat (mammalian homolog of BarH), is highly related to BarH2. It is a member of the BAR homeobox family of highly conserved proteins and contains one homeobox DNA-binding domain. Barhl2 localizes to the nucleus and is expressed in the central nervous system as well as in mature and differentiating amacrine, horizontal and ganglion cells of the retina. Barhl2 plays a role in regulating the specification of glycinergic amacrine cells and controls horizontal cell differentiation. Barhl2 may also be involved in the regulation of neural basic helix-loop-helix genes. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of Barhl2 of h and r origin by WB IP IF IHC(P) and ELISA |
|---|---|
| Tissue Specificity | recommended for detection of Barhl2 of h and r origin by WB, IP, IF, IHC(P) and ELISA |
| Entrez Gene | 343472 |
|---|
Application
Western blot analysis of Barhl2 expression in Hep G2 whole cell lysate.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human lung tissue showing nuclear localization.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human kidney tissue showing nuclear and cytoplasmic localization.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
