Application
Western blot analysis of Otoconin 90 expression in non-transfected 293: (A), human Otoconin 90 transfected 293: (B) and SK-MEL-28 (C) whole cell lysates.Application
Western blot analysis of Otoconin 90 expression in mouse brain tissue extract.| Product Name | Otoconin 90 Antibody (E-14) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping within an internal region of Otoconin 90 of human origin |
| Modification Notes | The ability to sense orientation relative to gravity requires dense particles, called otoconia, which are localized in the vestibular macular organs. In mammals, otoconia are composed of proteins (otoconins) and calcium carbonate crystals in a calcite lattice. Otoconin 90, also known as PLA2L (phospholipase A2 homolog) or OC90, is a 493 amino acid secreted protein belonging to the phospholipase A2 family. Consisting of three PA2-type domains, Otoconin 90 regulates the growth of otoconia crystals. The inertial mass of otoconia crystals provides a shearing force to stimulate the mechanoreceptors of the utricle and saccule (the gravity receptor organ) under the stimuli of linear motion. Otoconin 90 specifically recruits other matrix components, which are essential for formation of the organic matrix of otoconia. Otoconin 90 is encoded by a gene located on human chromosome 8, which consists of nearly 146 million base pairs, houses more than 800 genes and is associated with a variety of diseases and malignancies. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Otoconin 90 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping within an internal region of Otoconin 90 of human origin |
|---|---|
| Tissue Specificity | epitope mapping within an internal region of Otoconin 90 of human origin |
| Entrez Gene | 729330 |
|---|
Application
Western blot analysis of Otoconin 90 expression in non-transfected 293: (A), human Otoconin 90 transfected 293: (B) and SK-MEL-28 (C) whole cell lysates.
Application
Western blot analysis of Otoconin 90 expression in mouse brain tissue extract.| Application Notes | recommended for detection of Otoconin 90 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
